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empty vector ev control  (Vector Biolabs)


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    Structured Review

    Vector Biolabs empty vector ev control
    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Empty Vector Ev Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector+control+(ev/Ad-RFP/pm28764769-84-13-22
    Average 96 stars, based on 16 article reviews
    empty vector ev control - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis."

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    Journal: Arthritis research & therapy

    doi: 10.1186/s13075-017-1388-8

    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Figure Legend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Techniques Used: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay

    Related Articles

    Expressing:

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.
    Article Snippet: Primary TMJ cells were obtained as outgrowths from explants of TMJ condylar cartilage within 24 h after collection as 1-mm to 3-mm punch biopsies, and cultured in chondrocyte growth medium with 5% FBS (Lonza Inc.). .. Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs. .. These adenovirus particles were amplified in 293 T cells and quantified using an adenovirus quantification kit (Cell Biolabs).

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis
    Article Snippet: Primary TMJ cells were obtained as outgrowths from explants of TMJ condylar cartilage within 24 h after collection as 1-mm to 3-mm punch biopsies, and cultured in chondrocyte growth medium with 5% FBS (Lonza Inc.). .. Adenoviruses for transient expression of LOXL2 (Ad-CMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFP-LOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs. .. These adenovirus particles were amplified in 293 T cells and quantified using an adenovirus quantification kit (Cell Biolabs).

    Plasmid Preparation:

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.
    Article Snippet: Primary TMJ cells were obtained as outgrowths from explants of TMJ condylar cartilage within 24 h after collection as 1-mm to 3-mm punch biopsies, and cultured in chondrocyte growth medium with 5% FBS (Lonza Inc.). .. Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs. .. These adenovirus particles were amplified in 293 T cells and quantified using an adenovirus quantification kit (Cell Biolabs).

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis
    Article Snippet: Primary TMJ cells were obtained as outgrowths from explants of TMJ condylar cartilage within 24 h after collection as 1-mm to 3-mm punch biopsies, and cultured in chondrocyte growth medium with 5% FBS (Lonza Inc.). .. Adenoviruses for transient expression of LOXL2 (Ad-CMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFP-LOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs. .. These adenovirus particles were amplified in 293 T cells and quantified using an adenovirus quantification kit (Cell Biolabs).

    Control:

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.
    Article Snippet: Primary TMJ cells were obtained as outgrowths from explants of TMJ condylar cartilage within 24 h after collection as 1-mm to 3-mm punch biopsies, and cultured in chondrocyte growth medium with 5% FBS (Lonza Inc.). .. Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs. .. These adenovirus particles were amplified in 293 T cells and quantified using an adenovirus quantification kit (Cell Biolabs).

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis
    Article Snippet: Primary TMJ cells were obtained as outgrowths from explants of TMJ condylar cartilage within 24 h after collection as 1-mm to 3-mm punch biopsies, and cultured in chondrocyte growth medium with 5% FBS (Lonza Inc.). .. Adenoviruses for transient expression of LOXL2 (Ad-CMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFP-LOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs. .. These adenovirus particles were amplified in 293 T cells and quantified using an adenovirus quantification kit (Cell Biolabs).



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    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its <t>empty</t> <t>vector</t> (EV) <t>control</t> (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)
    Empty Vector Ev Control, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Journal: Arthritis research & therapy

    Article Title: Anabolic role of lysyl oxidase like-2 in cartilage of knee and temporomandibular joints with osteoarthritis.

    doi: 10.1186/s13075-017-1388-8

    Figure Lengend Snippet: Fig. 3 LOXL2 promotes a differentiated phenotype and inhibits apoptosis in human articular chondrocytes from cartilage affected by osteoarthritis (OA). a Human articular OA chondrocytes (HAC-OA) transduced with adenoviruses for transient expression of LOXL2 (Ad-RFP-LOXL2) compared to cells transduced with its empty vector (EV) control (Ad-FP-EV) did not affect chondrocyte proliferation after 24 h. b Lentiviral cytomegalovirus (CMV)-LOXL2 transduction of HAC-OA compared to CMV-EV increased the mRNA levels of collagen type II α1 (COL2A1) (P = 0.043), sex determining region Y-box containing gene 9 (SOX9) (*P < 0.001), and aggrecan (ACAN) (P = 0.002). c Ad-RFP-LOXL2 transduction inhibited caspase 3/7 activity induced by TNF-α or IL-1β (*P = 0.006) compared to Ad-RFP-EV in HAC-normal (HAC-N) and HAC-OA cells in serum-free medium after 24 h. d Adenoviral transduction (Ad-RFP-LOXL2) HAC-OA in serum-free medium increased COL2A1 mRNA levels (*P < 0.001) compared to control (Ad-RFP-EV) in the presence of TNF-α (*P = 0.001)

    Article Snippet: Adenoviruses for transient expression of LOXL2 (AdCMV-RFP-CMV-hLOXL2-His; referred to as Ad-RFPLOXL2), and its empty vector (EV) control (Ad-RFP-EV) were custom-synthesized (ADV-214438) by Vector Biolabs.

    Techniques: Transduction, Expressing, Plasmid Preparation, Control, Activity Assay